Influence of general vibration on the functions of the kidney mitochondrial respiratory chain of rabbits in the experiment

An experimental study of energy-dependent reactions of native rabbit kidney mitochondria under the adverse effect of various modes of general vertical vibration was conducted.

Energy-dependent reactions of native mitochondria of the cortical layer of the rabbit kidney before and after exposure to general vibration with a frequency of 8 and 44 Hz for 7, 21 and 56 sessions of 60 minutes, they were studied by polarographic method using a closed Clark-type membrane electrode. Various metabolic states of mitochondria were modeled in vitro by introducing exogenous energy substrates (succinic, glutamic, and malic acids) into the polarographic cell with tissue homogenate before and after the addition of the oxidative phosphorylation disconnector 2,4-dinitrophenol. The contribution to the endogenous respiratory activity of mitochondria of NAD — and FAD-dependent substrates was evaluated according to inhibitory analysis with amital or malonate.

The rate of endogenous respiration on the background of 8 Hz vibration ranged from 8.13±1.4 to 14.1±1.8 (ng-atom o) min-1mg-1, significantly differing from the same indicator of control animals after 56 sessions of vibration. Inhibitor analysis showed that vibration with a frequency of 44 Hz in the same time period caused an increase in malonatosensitivity by 40% (p<0.05) with its subsequent decrease below the control level, indicating the beginning of suppression of succinate-dependent bioenergetics.

The oxidation of exogenous NAD-dependent substrates (malic and glutamic acids) is suppressed regardless of the frequency of vibration, while the rate of oxidation of exogenous succinic acid increases by 45% (p<0.05) after 21 sessions of 44 Hz vibration, decreasing to the end of 56 sessions of vibration. Similar changes were observed in the disconnected state of the respiratory chain of mitochondria, as evidenced by multidirectional high-amplitude fluctuations of the VJ-p index in the range of 50-60% relative to the control level after 7, 21 and 56 sessions of vibration exposure.

It was found that the imbalance between the functional activity of FAD-and NAD-dependent links of the respiratory chain of mitochondria depends on the frequency and duration of vibration, indicates the development of bioenergetic hypoxia and is accompanied by morphohistological signs of glomerulopathy of exudative intra- and extra-capillary type.

1. Izmerov N.F., Buhtiyarov I.V., Prokopenko L.V., Shigan E.E. Implementation of the WHO global action plan for the health of workers in the Russian Federation. Med. truda i prom. ekol. 2015; 9: 4-10 (in Russian).

2. Ando H., Noguchi R., Ishitake T. Frequency dependence of hand-arm vibration on palmar sweating response. Scand. J. Work Environ. Health. 2002; 28 (5): 324-7.

3. Rahimov Ya.A., Sapin M.R., Belkin V.Sh., Etingen L.E. Morphology of internal organs under the action of vibration. Dushanbe, Vysshaya shkola; 1979 (in Russian).

4. Saarkoppel’ L.M., Kir’yakov V.A., Oshkoderov O.A. The role of modern biomarkers in the diagnosis of vibrational disease. Med. truda i prom. ekol. 2017; 2: 6-10 (in Russian).

5. Saxton J.M. A review of current literature on physiological tests and soft tissue biomarkers applicable to work-related upper limb disorders. Occup. Med. 2000; 50 (2): 121-30.

6. Zueva M.A., Shpagina L.A., Gerasimenko O.N., Zyubina L.Yu. Mihno I.P. Hemodynamic and microcirculatory mechanisms of the formation of liver damage in vibratory disease. Med. truda i prom. ekol. 2010; 8: 14-9 (in Russian).

7. Suharevskaya T.M., Efremov A.V., Nepomnyashchih G.I., Loseva M.I. et al. Microangiopathy and visceropathy with vibrational disease. Novosibirsk; 2000 (in Russian).

8. Smirnova E.L., Poteryaeva E.L., Nikiforova N.G. Individual characteristics of lipid peroxidation and antioxidant protection in individuals with vibrational disease in the post-exposure period. Med. truda i prom. ekol. 2010; 8: 36-40 (in Russian).

9. Korzeneva E.V., Sineva E.L. Diseases of the cardiovascular system in workers of leading professions in the mining and engineering industries. Med. truda i prom. ekol. 2007; 10: 27-31 (in Russian).

10. Garipova R.V., Strizhakov A.A., Arhipov E.V. Occupational kidney damage from exposure to physical and biological factors. Med. truda i prom. ekol. 2019; 1: 38-44 (in Russian).

11. Zaharchenko M.V., Hunderyakova N.V., Kondrashova M.N. The importance of preserving the biophysical organization of the isolated mitochondria to identify the physiological regulation of their function. Biofizika. 2011; 56 (5): 840-47 (in Russian).

12. Kondrashova M.N. Instrumentation and procedure for po-larographic measurement of mitochondrial respiration. Guide to the study of biological oxidation by the polarographic method. Ed. M.N. Kondrashova. M.: Nauka; 1973 (in Russian).

13. Vorob’eva VV, Shabanov P.D. A vibrational model of the hypoxic type of cell metabolism evaluated on rabbit cardiomyo-cytes. Byulleten eksperimental'noj biologii i mediciny. 2009; 147 (6): 712-5 (in Russian).

14. Nikol’s D. Bioenergy. Introduction to chemosmotic theory. Mir; Moskva; 1985 (in Russian).

15. Goa J. A micro biuret method for protein determination. Determination of total protein in cerebrospinal fluid. Scand. J. Clin. Lab. Invest. 1953; 5: 218-22.

16. Maevskij E.I., Grishina E.V., Rozenfel’d A.S., Zyakun A.M. Anaerobic formation of succinate and facilitation of its oxidation are possible mechanisms of cell adaptation to oxygen starvation. Biofizika. 2000; 45 (3): 509-13 (in Russian).

17. Luk’yanova L.D. Problems of hypoxia: molecular, physiological and medical aspects. M.: Meditsina; 2004 (in Russian).

18. Vorob’eva V.V., Shabanov P.D. Morphofunctional changes in the rabbit myocardium when exposed to general vibration and after pharmacological protection with succinic acid. Biofizika. 2019; 64 (2): 337-42. DOI: 10.1134/S000630919020210 (in Russian).

19. Vorob’eva V.V., Shabanov P.D. Morphofunctional changes in the rabbit myocardium when exposed to general vibration and after pharmacological protection with succinic acid. Vestnik SPbGU. 2010; (3): 201-7 (in Russian).

20. Matoba T. Pathophysiology and clinical pucture of hand-arm vibration syndrome in Japanes workers. Nagoya J. Med. Sci. 1994; 57: 19-26.

21. Griffin M.J., Bovenzi M. Dose-responte patterns for vibration-induced white figner. Occup. Environ. Med. 2003; 60 (1): 16-26.

22. Grigor’ev A.I., Tonevickij A.G. Molecular mechanisms of stress adaptation: early response genes. Rossijskij fizio-logicheskij zhurnal im. I.M. Sechenova. 2009; 95 (10): 1041-57 (in Russian).

23. Stroka D.M., Burkhardt T., Desballerts I. HIF — 1 is expressed in normoxia tissue and displays an organ — specific regulation under systemic hypoxia. FASEB J. 2001; 15: 2445- 53.

24. Semenza G.L. Expression of hypoxia-inducible factor 1: mechanisms and consequences. Bioch. Pharmacol. 2000; (59): 47-53.

25. Vorob’eva VV., Shabanov P.D. The protective properties of reamberin in the acute combined action of cold, vibration and immobilization in the experiment. Med. truda iprom. ekol. 2018; (4): 47-50 (in Russian).