Comparative assessment of morphological mechanisms of maintaining structural liver homeostasis in the dynamics of exposure to coal-rock dust and sodium fluoride on the body

Introduction. The impact on the body of such factors of the production environment as coal-rock dust and fluorine compounds leads to certain shift s in strict indicators of homeostasis at the system level. Maintaining the relative constancy of the internal environment of the body is provided by the functional consistency of all organs and systems, the leading of which is the liver. Organ repair plays a crucial role in restoring the structure of genetic material and maintaining normal cell viability. When this mechanism is damaged, the compensatory capabilities of the organ are disrupted, homeostasis is disrupted at the cellular and organizational levels, and the development of the main pathological processes is noted.

The aim of the study is to compare the morphological mechanisms of maintaining structural homeostasis of the liver in the dynamics of the impact on the body of coal-rock dust and sodium fluoride.

Materials and methods. Experimental studies were conducted on adult white male laboratory rats. Features of morphological mechanisms for maintaining structural homeostasis of the liver in the dynamics of exposure to coal-rock dust and sodium fluoride were studied on experimental models of pneumoconiosis and fluoride intoxication. For histological examination in experimental animals, liver sampling was performed after 1, 3, 6, 9, 12 weeks of the experiment.

Results. The specificity of morphological changes in the liver depending on the harmful production factor was revealed. It is shown that chronic exposure to coal-rock dust and sodium fluoride is characterized by the development of similar morphological changes in the liver and its vessels from the predominance of the initial compensatory-adaptive to pronounced violations of the stromal and parenchymal components. Long-term inhalation of coal-rock dust at 1–3 weeks of seeding triggers adaptive mechanisms in the liver in the form of increased functional activity of cells, formation of double-core hepatocytes, activation of immunocompetent cells and endotheliocytes, ensuring the preservation of the parenchyma and the general morphostructure of the organ until the 12th week of the experiment. Exposure to sodium fluoride leads to early disruption of liver compensatory mechanisms and the development of dystrophic changes in the parenchyma with the formation of necrosis foci as early as the 6th week of the experiment.

Conclusions. The study of mechanisms for compensating the liver structure in conditions of long-term exposure to coal-rock dust and sodium fluoride, as well as processes that indicate their failure, and the timing of their occurrence, is of theoretical and practical importance for developing recommendations for the timely prevention and correction of pathological conditions developing in employees of the aluminum and coal industry.

The authors declare no conflict of interests.

1. Artamonova V.G., Fishman B.B., Lashina E.L. Features of the immune response in workers exposed to silicate dust. In: Izmerov N.F., Chuchalin A.G. eds. Occupational respiratory diseases: national guidelines (National Guides Series). Moscow: GEOTAR-Media; 2015: 220–232 (in Russian).

2. Korotenko O.Yu., Panev N.I., Zakharenkov V.V., Filimonov S.N., Semenova E.A., Panev R.N. Chronic fluoride intoxication as a risk factor for the development of atherosclerosis. Gigiena i sanitariya. 2015; 94(5): 91–4 (in Russian).

3. Nenenko O.I., Serebryakov P.V., Rakhimzyanov A.R., Khoshtariya N.V. Assessment of the adaptive capabilities of the cardiorespiratory system in workers in dust occupations. Med. truda i prom. ekol. 2015; (9): 102 (in Russian).

4. Podymova S.D. Liver disease. Moscow: Meditsinskoe informatsionnoe agentstvo; 2018 (in Russian).

5. Kuznetsova E.E., Gorokhova V.G., Dorofeev A.S. The role of the liver in chemical homeostasis and the control of its detoxification function using an antipyrine test. Izvestiya vuzov. Prikladnaya khimiya i biotekhnologiya. 2014; 7(2): 58–62 (in Russian).

6. Chesnokova N.P., Ponukalina E.V., Polutova N.V. The role of the liver in ensuring the constancy of protein and carbohydrate metabolism in the body due to the functional interaction with endocrine and nervous influences under normal conditions (Article 2): the role of the liver in protein metabolism. In: The concept of the “Knowledge Society” in modern science: Proceedings of the International Scientific and Practical Conference. Perm; 2019: 171–6 (in Russian).

7. Lyzikov A.N., Skuratov A.G., Osipov B.B. The mechanisms of liver regeneration are normal and with pathology. Problemy zdorov’ya i ekologii. 2015; 43(1): 4–9 (in Russian).

8. Karkishchenko N.N. Biomodeling basics. Moscow: Izd-vo VPK; 2004 (in Russian).

9. Sosedova L.M. Methodological approaches to the experimental study of the effect of pollution of environmental objects on the human body. Gigiena i sanitariya. 2014; 93(6): 94–8 (in Russian).

10. Gorokhova L.G., Bugaeva M.S., Ulanova E.V., Fomenko D.V., Kizichenko N.V., Mikhaylova N.N. A method for seeding laboratory animals with industrial dust for modeling silicosis. Patient № 2546096; 2015 (in Russian).

11. Ulanova E.V., Mikhaylova N.N., Danilov I.P., Anokhina A.S., Fomenko D.V., Kizichenko N.V. A method for the prevention of chronic fluoride osteopathy when modeling fluoride intoxication in an experiment. Patient № 2300374; 2007 (in Russian).

12. Mikhaylova N.N., Sazontova T.G., Alekhina D.A., Kazitskaya A.S., Zhdanova N.N., Prokop’ev Yu.A. et al. Features of intracellular defense mechanisms when various xenobiotics act on the body. Tsitokiny i vospalenie. 2013; 12(4): 71–5 (in Russian).

13. Kazitskaya A.S. Experimental studies of the effects of harmful production factors on the immune status of an organism. In: Profession and Health: Materials of the 1st International Youth Forum. Moscow: AMT, FGBNU «NII MT»; 2016: 55–7 (in Russian).

14. Zavodnik I.B. Mitochondrial dysfunction and compensatory mechanisms in liver cells in acute toxicity of rats with carbon tetrachloride. Biomeditsinskaya khimiya. 2015; 61(6): 731–6 (in Russian).

15. Zuo H., Chen L., Kong M., Qiu L., Lü P., Wu P. et al. Toxic effects of fluoride on organisms. Life Sci. 2018; 198: 18–24.

16. Araujo T.T., Barbosa Silva Pereira H.A., Dionizio A., Sanchez C.D.C., de Souza Carvalho T., da Silva Fernandes M. et al. Changes in energy metabolism induced by fluoride: Insights from inside the mitochondria. Chemosphere. 2019; 236: 124357.

17. Kurzeja E., Pawłowska-Góral K., Burzyński M., Bycina K. The effects of exposure time and fluorine concentration on cell homeostasis. Med Środow. 2015; 18(3): 12–6.

18. Zhukova A.G., Mikhaylova N.N., Yadykina T.K., Alekhina D.A., Gorokhova L.G., Romanenko D.V. et al. Experimental studies of the intracellular defense mechanisms of the liver in the development of chronic fluoride intoxication. Med. truda i prom. ekol. 2016; (5): 21–4 (in Russian).