Immunoassay of antibodies to benzo[a]pyrene for lung cancer risk diagnosis among coal-mining workers
https://doi.org/10.31089/1026-9428-2019-3-174-177
Abstract
Th e authors detected association of G-class antibodies specifi c to chemical carcinogen benzo[a]pyrene (IgG-Bp) with lung cancer in healthy men not working at coal mines. Objective — to reveal possibilities of immunoassay of IgG-Bp in evaluating risk of lung cancer in coal mine workers. Th e study covered serum IgG-Bp in 501 miners aged under 60 (161 healthy, not working in mine; 239 miners; 101 patients with lung cancer), by means of non-concurrent immune-enzyme analysis using benzo[a]pyrene conjugates with bovine serum albumin as an antigen. Findings are that increased levels of IgG-Bp in lung cancer patients are more frequent (50.5%) than in healthy men not working in mine (37.2%, p=0.047, OR=1.7) and in miners (30.5%, p=0.0008, OR=2.3). Length of service in mines did not infl uence serum levels of IgG-Bp in coal industry workers. In the miners, with age adjustment consideration, OR for lung cancer equaled 2.0 (p=0.01), and with adjustment for smoking OR=2.3 (p=0.001). Occupational (work in mine), lifestyle (smoking) and endogenous (age) factors do not have signifi cant infl uence on intensity of individual specifi c reaction to benzo[a]pyrene in males. Immunoassay of IgG-Bp is recommended for lung canc er diagnosis in coal mine workers.
About the Authors
A. N. Glushkov
Federal Research Center of Coal and Coal Chemistry
Russian Federation
Russian Federation
E. G. Polenok
Federal Research Center of Coal and Coal Chemistry
Russian Federation
Russian Federation
M. V. Kostyanko
Kemerovo State University
Russian Federation
Russian Federation
V. A. Titov
Regional Clinical Oncology Dispensary
Russian Federation
Russian Federation
I. A. Vafin
Regional Center of Blood
Russian Federation
Russian Federation
References
1. Attfield M.D., Kuempel E.D. Mortality among U.S. underground coal miners: a 23-year follow-up. Am. J. Ind. Med. 2008; 51 (4): 231–45.
2. Laney A.S., Weissman D.N. Respiratory diseases caused by coal mine dust. J. Occup. Environ. Med. 2014. 56 (10): S18–22.
3. Hosgood H.D. 3rd, Chapman R.S., Wei H. et al. Coal mining is associated with lung cancer risk in Xuanwei, China. Am. J. Ind. Med. 2012; 55 (1): 5–10.
4. Hoffmann B., Jöckel K.H. Diesel exhaust and coal mine dust: lung cancer risk in occupational settings. Ann. N. Y. Acad. Sci. 2006; 1076: 253–65.
5. Miller B.G., MacCalman L. Cause-specific mortality in British coal workers and exposure to respirable dust and quartz. Occup. Environ. Med. 2010; 67 (4): 270–6.
6. Skowronek J., Zemla B. Epidemiology of lung and larynx cancers in coal mines in Upper Silesia--preliminary results. Health Phys. 2003; 85 (3): 365–70.
7. Lee M.S., Liu C.Y., Su L., Christiani D.C. Polymorphisms in ERCC1 and ERCC2/XPD genes and carcinogen DNA adducts in human lung. Lung Cancer. 2015; 89 (1): 8–12.
8. Pauk N., Klimesova S., Kara J. et al. The relevance of monitoring of antibodies against polycyclic aromatic hydrocarbon (PAH) and PAH-DNA adducts in serum in relation to lung cancer and chronic obstructive pulmonary disease (COPD). Neoplasma. 2013; 60 (2): 182–7.
9. Petruzzelli S., Celi A., Pulera N. et al. Serum antibodies to benzo(a) pyrene diol epoxide DNA adducts in the general population: effects of air pollution, tobacco smoking and family history of lung diseases. Cancer Res. 1998; 58 (8): 4122–6.
10. Reid M.E., Santella R., Ambrosone C.B. Molecular epidemiology to better predict lung cancer risk. Clin. Lung Cancer. 2008; 9 (3): 149–53.
11. Glushkov A.N., Polenok E.G., Kostyanko M.V., Titov V.A., Vafin I.A., Ragozhina S.E. Mutual effects of antibodies to benzo[a]pyrene, estradiol and progesterone on the lung cancer risks. Rossijskij immunologicheskij zhurnal. 2015; 9(18 (3)): 343–9 (in Russian).
12. Glushkov A.N., Polenok E.G., Anosova T.P., Savchenko Ya.A., Bakanova M.L., Minina V.I., Mun S.A., Larin S.A., Kost’anko M.V. Serum antibodies to benzo[a]pyrene and chromosomal aberrations in lymphocytes peripheral blood at the workers of coal processing enterprise. Rossijskij immunologicheskij zhurnal. 2011. 5(14 (1)): 39–44 (in Russian).
13. Hajian-Tilaki K. Receiver Operating Characteristic (ROC) Curve Analysis for Medical Diagnostic Test Evaluation. Caspian J. Intern. Med. 2013: 4 (2): 627–35.
Review
For citations:
Glushkov A.N., Polenok E.G., Kostyanko M.V., Titov V.A., Vafin I.A. Immunoassay of antibodies to benzo[a]pyrene for lung cancer risk diagnosis among coal-mining workers. Russian Journal of Occupational Health and Industrial Ecology. 2019;(3):174-177. (In Russ.) https://doi.org/10.31089/1026-9428-2019-3-174-177
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