Experimental study of quantitative and corpuscular properties of rat’s leukocytes under the influence of ore
https://doi.org/10.31089/1026-9428-2023-63-8-551-557
EDN: gxexpk
Abstract
Introduction. Mining industry workers are at risk, as heavy metal compounds that are part of copper-zinc pyrite ore can accumulate in their bodies. One of the sensitive parts of the body to the action of heavy metals is blood, in which leukocytes play a leading role.
The study aims to explore the effect of copper-zinc pyrite ore on the quantitative and corpuscular properties of peripheral blood leukocytes.
Materials and methods. A group of researchers have conducted an experimental study on 48 white mongrel male rats aged 3–4 months, weighing 210.5±10.5 g. For 30 days, an aqueous suspension of ore powder was orally administered to rats daily at a dose of 600 mg/kg of animal body weight. In the ore sample under study, the proportion of lead and cadmium was 0.060% and 0.009%, respectively. In accordance with the conditions of the experiment , the scientists have divided the rats into the following groups: a control group in which the rats did not receive ore; an experimental group in which rats received ore for 10 days; an experimental group in which rats received ore for 20 days; an experimental group in which rats received ore for 30 days. The researchers measured blood parameters using a veterinary semi-automatic hematology analyzer Vet Exigo 19 (Sweden), which determined the following parameters of leukocytes: total number of leukocytes, absolute number of lymphocytes, monocytes and granulocytes. We calculated the average volume of lymphocytes, granulocytes and monocytes based on histograms of the distribution of cells by volume issued by the device. To assess the statistical significance of the differences in variables, the authors used the Mann–Whitney U-test.
Results. In the experimental group of animals after 30 days of ore intoxication, the average volume and number of granulocytes and monocytes increased statistically compared to the control. The authors have revealed a correlation between the parameters of cell distribution and the average volume in leukocyte populations at different times of ore intoxication.
Conclusion. The effect of copper-zinc pyrite ore on the condition of the leukocyte link of peripheral blood was an increase in the average population volumes of all types of leukocytes by the 30th day of the experiment, due to a change in the ratio of the number of cells of different sizes and the degree of heterogeneity of the populations.
Limitations. The limitation of this study is the model of oral administration of copper-zinc pyrite ore in the form of an aqueous suspension.
Ethics. The authors have conducted the study in compliance with the "Rules of work using experimental animals". The study was approved by the local Ethics Committee of the Bashkir State Medical University, Ministry of Health of the Russian Federation, Protocol No. 5 of 13.09.2017.
Contribution:
Ayupova A.R. — the concept and design of the study, data collection and processing, the text writing;
Ziyakaeva K.R. — data collection and processing, the text writing;
Kayumova A.F. — the concept and design of the study, the editing;
Shamratova V.G. — the editing.
Gratitude. The authors express their gratitude to Valentin Nikolaevich Pavlov, Academician of the Russian Academy of Sciences, Rector of the Bashkir State Medical University of the Ministry of Health, Russian Federation, for his assistance in conducting the study.
Funding. The study had no funding.
Conflict of interests. The authors declare no conflict of interests.
Received: 26.06.2023 / Accepted: 18.08.2023 / Published: 05.09.2023
About the Authors
Albina R. Ayupova
Bashkir State Medical University
Russian Federation
Russian Federation
Klara R. Ziyakaeva
Bashkir State Medical University
Russian Federation
Russian Federation
The Associate Professor at the Department of Normal Physiology of the Bashkir State Medical University, Cand. of Sci. (Biol.).
e-mail: klazia@yandex.ru
Aliya F. Kayumova
Bashkir State Medical University
Russian Federation
Russian Federation
Valentina G. Shamratova
Bashkir State Medical University
Russian Federation
Russian Federation
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Review
For citations:
Ayupova A.R., Ziyakaeva K.R., Kayumova A.F., Shamratova V.G. Experimental study of quantitative and corpuscular properties of rat’s leukocytes under the influence of ore. Russian Journal of Occupational Health and Industrial Ecology. 2023;63(8):551-557. (In Russ.) https://doi.org/10.31089/1026-9428-2023-63-8-551-557. EDN: gxexpk
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