Informative laboratory biomarkers for the diagnosis of respiratory diseases of occupational etiology in modern conditions

Chronic obstructive pulmonary disease (COPD) is one of the most serious problems of modern healthcare in the world. COPD is a common disease, characterized by a variety of clinical and functional features of the disease and heterogeneity of the inflammatory response.

The study aims to research a comprehensive analysis of laboratory examination in persons at high risk of COPD, including data from general clinical and bacteriological studies and highlighting priority, early changes in its composition.

The researchers analyzed the results of laboratory studies of the mucous membranes of the upper respiratory tract in persons with an established diagnosis of chronic obstructive pulmonary disease. The work uses data from official reports of the Ministry of Health for 2020.

When evaluating the general (native) analysis of sputum in COPD patients, the nature was determined by its cellular composition. Segmented neutrophils predominated among leukocytes in 41.18±5.37%, eosinophils in 8.24±3.00%, alveolar macrophages in 9.41±3.19%. We found that Streptococcus pyogenes, Staph. epidermidis, Enterococcus spp., Candida albicans prevailed among the isolated microorganisms during the exacerbation of this disease. The remaining types of microorganisms (Klebsiella pnemoniae, Enterobacter aeromonas, Escherichia coli) were represented by single strains.

The results of studies on the cellular composition and microbial spectrum of sputum of patients with acute COPD, allow us to conclude that the most informative and significant are general clinical indicators (leukocytes, eosinophils), as well as isolated microorganisms Streptococcus pyogenes, Staph. epidermidis, Enterococcus spp., and yeast fungi of the genus Candida albicans. The data obtained made it possible to substantiate the role of reliable and easily reproducible biomarkers in determining the activity of the disease and the phenotype of COPD.

Contribution:
Vlasova N.V. — concept and design of the study, collection and processing of material, writing the text;
Karamova L.M. — editing;
Rafikova L.A. — concept and design of the study, collection and processing of material, writing the text;
Gizatullina L.G. — compilation of the list of references;
Abdrakhmanova E.R. — collection and processing of material;
Borisova A.I. — collection and processing of material.
All co–authors — approval of the final version of the article, responsibility for the integrity of all parts of the article.

Funding. The study had no funding.

Conflict of interests. The authors no conflict of interests.

Received: 25.11.2022 / Accepted: 28.11.2022 / Published: 23.12.2022

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