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Study of photobiomodulation effects with violet-blue and red light in experimental oncogenesis

https://doi.org/10.31089/1026-9428-2020-60-9-618-623

Abstract

Introduction. There is still an open question about the limits of medical use of low-intensity electromagnetic radiation of the optical range in tumor growth due to the risk of increased proliferation of tumor cells. The conditions under which the tumor process is stimulated, as well as the mechanisms of photobiomodulation in oncological pathology, remain unclear.

The aim of the study - in vitro evaluation and comparison of the effect of low-intensity electromagnetic radiation with wavelengths of 400, 460 and 660 nm on the activity of free-radical processes in tumor tissue and blood in normal and growing experimental neoplasia. Materials and methods. The study was conducted on biological material obtained from white non-linear rats intact and with subcutaneously transplanted cholangiocellular cancer MS-1. The sources of low-intensity radiation with wavelengths of 400, 460 and 660 nm were led generators. The content of hemoglobin, the activity of superoxide dismutase and catalase, changes in the overall level of free radical processes and antioxidant activity by induced chemiluminescence, and DNA damage by the method of DNA comets were studied. Data analysis was performed using nonparametric statistics methods.

Results. The multidirectional effect of radiation with wavelengths of 400, 460 and 660 nm on free-radical homeostasis indicators at the early and late stages of tumor growth, as well as the dependence of biological effects on the wavelength of radiation, was found. Conclusions. The results obtained allow making a number of assumptions about the mechanisms of action of the optical electromagnetic waves on tumor growth, modulating free radical processes in the tumor-bearing organism.

About the Authors

Evgeniya S. Zhukova
Nizhny Novgorod Research Institute for Hygiene and Occupational Pathology
Russian Federation

Junior researcher of the department of medical and preventive technologies of managing public health risks, Nizhny Novgorod Research Institute of Hygiene and Occupational Pathology.

e-mail: medprof_otd@nniigp.ru



Tatiana G. Shcherbatyuk
Nizhny Novgorod Research Institute for Hygiene and Occupational Pathology; Pushchino State Institute of Natural Science; Moscow Region State University
Russian Federation


Arseniy L. Potapov
Privolzhsky Research Medical University
Russian Federation


Irina A. Chernigina
Privolzhsky Research Medical University
Russian Federation


Vladimir V. Chernov
Institute of Applied Physics of the Russian Academy of Sciences
Russian Federation


Andrew B. Gapeyev
Moscow Region State University; Institute of Cell Biophysics of the Russian Academy of Sciences
Russian Federation


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Review

For citations:


Zhukova E.S., Shcherbatyuk T.G., Potapov A.L., Chernigina I.A., Chernov V.V., Gapeyev A.B. Study of photobiomodulation effects with violet-blue and red light in experimental oncogenesis. Russian Journal of Occupational Health and Industrial Ecology. 2020;60(9):618-623. (In Russ.) https://doi.org/10.31089/1026-9428-2020-60-9-618-623

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ISSN 1026-9428 (Print)
ISSN 2618-8945 (Online)