Evaluation of the ef ectiveness of alimentary correction of pathological changes in hepatic tissue under the inf uence of industrial copper-containing dust in the experiment

The results of comparative studies of the long-term ef ect (dynamics) of polymetallic dust containing 0.6% copper in the shops of Balkhash mining and metallurgical combine on the liver of rats are presented. These data on the action of dust with a predominant copper content of 0.6% give reason to assert that in the liver for 30 days there were changes in the structure, which were ref ected by metabolic rearrangements and are characterized as a failure of adaptation. The phenomena of hepatitis also took place on day 90, but these changes led to a decrease in the rate of metabolic processes. Microscopic examination showed that long-term exposure to polymetallic dust with a copper concentration of 0.6% in the body leads to signif cant changes in the liver reactive character, followed by transformation into hepatitis portal type. There is an accumulation of copper in the cytoplasm of hepatocytes; on day 90, the number of dust particles in rat hepatocytes increases by 2.25 times compared to 30 days of the experiment. T is can be explained by the inhibition of the phagocytic function of liver macrophages and ways to eliminate dust from the body. The lat er, in turn, leads to functional and metabolic damage to the structural component of the organ. It should be noted that an important role in the neutralization and removal of copper from the cell belongs to lysosomes. T ere is an assumption that copper damages lysosomal membranes and stimulates the release of enzymes from lysosomes due to a decrease in the number of mitochondria in the cell or inhibition of their enzymes.
Thus, the ef ects of polymetallic dust with a copper concentration of 0.6% in the body in the early stages leads to changes in the liver, in the form of reactive hepatitis with subsequent transformation into portal-type hepatitis. The use of alimentary corrections slows down the development of post-necrotic f brosis in the liver, and there was a signif cant decrease in the volume fraction of f brotic liver tissue.

1. Guselnikov S. R., Gogoleva O. I., Lipatov G. Ya., Adrianovsky V. I., Samylkin A. A. Professional morbidity of workers engaged in obtaining ref ned copper. Med. truda i prom. ekol. 2015; 9: 46–47 (in Russian).

2. Lozova E.V., Karimova L.K., Gainullina M.K., Mavrina L.N., Bagul N. Ah. Salimgareeva T. M. Hygienic assessment of working conditions of workers of mining and processing plants. Med. truda i prom. ekol. 2015; 3: 121–127 (in Russian).

3. Sadykov M. N., Otarov E. Zh., Asenova L. X., Makanova U.K., et al. Assessment of working conditions of mining workers. Med. truda i prom. ekol. 2017; 3: 71–73 (in Russian).

4. Serebryakov P.V., Kartashev O.I., Fedina I.N. Clinical and hygienic assessment of the health of copper production workers in the Far North. Med. truda i prom. ekol. 2016; 1: 25–28 (in Russian).

5. Lipatov G.Ya., Adrianovsky V.I., Konstantinov V.G., Gilyova Yu.M. Mortality from malignant neoplasms of workers engaged in the enrichment of copper-containing ores. Ural honey. zhur. 2010; 1(66): 5–7.

6. Izmerov NF, Kovalevsky E.V., Rukavishnikov V.S. Industrial dust. Aerosols predominantly firogenic action. In the book: Izmerov N.F., Chuchalin A.G. (Ed.) Occupational respiratory diseases. M.: GEOTAR-Media; 2015:44–58 (in Russian).

7. Kavasaki HA. Mechanistic review of silica-indused inhalation toxicity. Inhal Toxicol. 2015;27(8):363–377 (in Russian).

8. Pankova V.B. Current problems of diagnosis and examination of occupational diseases of the upper respiratory tract. Bulletin of Otorhinolaryngology. 2015;(5):14–18 (in Russian).

9. Shorin S. S. Environmental factors of industrial cities that worsen the health of the population, and ways to solve them. Vectnik Karagandinskogo univerciteta. Seria Biologia. Medicina. Giograf a. 2014; 2: 35–43. (in Kazakhstan)

10. Farshatova E. R. Menshikova I. A., Kamilov F. Kh. T e inf uence of the metals contained in copper-zinc sulf de ores, on bone metabolism. Medicinsci vectnic Bashkortostan. 2014; 4: T 9: 57–59 (in Russian).

11. Pavlov V.N., Bekmukhambetov E. Zh., Teregulova Z. S., et al. To the evaluation of reproductive health of men living and working in the conditions of mining technogenesis. Medicinsci vectnic Bashkortostan. 2015; 3: T. 10: 103–105 (in Russian).

12. Tataeva R. K., Mussina A. A., Burumbaeva M. B. Morphological characteristics of liver tissue inhalation polymetallic aerosol condensation. News of the national academy of sciences of the republic of Kazakhstan series of biological and medical. 2014; 4(302): 43–46. (in Kazakhstan)

13. Ibrayeva L. K., Bat akova J. E., Amanbekova A. U., Chanturia G. R. Тhe character of energy changes in the activity of enzymes in the blood of rats under the action of f nely dispersed aerosols of polymetallic dust and alimentary correction. Fundamentalnaya issledovania. 2011; 9 (part 2): 251–253 (in Russian).

14. Shaimardanova G. M. Peroxidation in the lungs, liver and kidneys of rats with chronic exposure of polymetallic dust. Uspechy sovremennogo ectectvozhnania. 2008; 8: 19–22; (in Russian)

15. Kuz’mina L.P., Izmerova N.I., Burmistrova T.B., Druzhinin V.N. i dr. Patomorfoz sovremennyh form professional’nyh zabolevanij. Med. tuda i prom. Jekol. 2008; 6: 18–24 (in Russian).

16. Kupsha E.I. Morphofunctional characteristic of the nuclei of hepatocytes of mice with lead intoxication. Mezhdunarodnyy nauchno-issledovatel’skiy zhurnal 2017; 11–3: 27–2 (in Russian)

17. Kozlakova N. In. Manual feeding of laboratory animals, Guinea pig and poultry producers. M.; 1968 (in Russian).