Features of chemical occupational hazards influence on immune system ofworkers engaged into organic synthesis production

Examination covered workers of enterprise producing phthalic anhydride and fumaric acid (n = 104). Phthalic acid was detected in serum of main group workers (phthalic and fumaric acids in air of workplace) and in 100% of the examinees in concentrations range 0.077-0.420 mg/dm³, in reference group workers (absence of occupational hazard) - in 71% of the examinees in concentrations range 0.005-0.154 mg/dm³. Fumaric acid in serum of reference group workers was seen in 57% of the examinees in concentrations range 0.001-0.344 mg/dm³, in the reference group - in 26% of the examinees in concentrations range 0.002-0.104 mg/dm³. Average group concentrations (p < 0.05) of phthalic and fumaric acids in serum of main group workers were 2.6 and 7.6 times higher respectively. Reliable decrease of CD95 receptors of T-cells expression was also revealed. Allergic sorbent testing demonstrated increase in specific sensibilization to phthalic anhydride and its derivative phthalic acid in 79.2% of the workers, maximal average group concentrations of IgE to phthalic anhydride were detected in serum of mechanical technicians and operators of crystallization, absorption and chemical reagents preparation. Mathematic model of logistic regression describing dependence “serum level of phthalic acid - specific IgE to phthalic anhydride level” (exposure - response probability) obtained parameters - b₀ = 0.038; b₁ = 4.064; R² = 0.18; F = 19.568; p<0.01, that prove reliability of the model established. Immune regulation parameters: CD95+, TNFR, pro-apoptotic factor bax, anti-apoptotic protein Bcl-2, IgE specific to phthalic anhydride - describe immune regulation in workers of phthalic anhydride production and can be recommended as effect markers in evaluation of health risk for workers exposed to organic synthesis products.

воздух рабочей зоны, фталевый ангидрид, фталевая кислота, фумаровая кислота, IgE специфический к фталевому ангидриду, air of workplace, phthalic anhydride, phthalic acid, fumaric acid, IgE specific to phthalic anhydride

1. Биологический энциклопедический словарь / гл. ред. М.С. Гиляров. - М.: Советская энциклопедия, 1986. - 831 с.

2. Долгих О.В., Зайцева Н.В., Кривцов А.В., Старкова К.Г., Дианова Д.Г., Бубнова О.А., Отавина Е.А., Безрученко Н.В. Разработка методических подходов к идентификации особенностей генетического полиморфизма и экспрессии генов у детей в условиях воздействия химических средовых факторов на примере стронция // Анализ риска здоровью. - 2016. - №1. - P. 34-41.

3. Салимгареева Т.М., Каримова Л.К., Бейгул Н.А., Маврина Л.Н., Гимаева З.Ф. Гигиенические аспекты оценки условий труда и их влияние на здоровье работников, занятых в производстве фталевого ангидрида // Пермский мед. ж-л. - 2015. - Т. XXXII, № 4. - С. 92-96.

4. Cyclic acid anhydrides: human health aspects. Concise International Chemical Assessment Document 75 // World Health Organization. - 2009. - Available at: www. inchem. org/documents/cicads/cicads/cicad75.pdf.

5. Lefevere M.F., Verhaeghe B.J., DeclerckD.H., Van Bocxlaer J.F., De Leenheer A.P. Metabolic Profiling of Urinary Organic Acids by Single and Multicolumn Capillary Gas Chromatography // J. Chromatogr. Sci. - 1989. - 27(1). - Р. 23-29.

6. Pfаffli P. Phthalic acid excretion as an indicator of exposure to phthalic anhydride in the work atmosphere // Int. Arch. Occup. Environ. Health. - 1986. - 58. - P. 209-216.

7. Phthalic anhydride. SIDS Initial Assessment Report. - Paris, France. - 2005. - P. 19-22. Available at: https:// ru. scribd. com/document/168865109/ PHTHALIC-ANHYDRIDE-pdf.