zurniimtpeМедицина труда и промышленная экологияRussian Journal of Occupational Health and Industrial Ecology1026-94282618-8945FSBSI “Izmerov Research Institute of Occupational Health”10.31089/1026-9428-2019-59-10-860-865zurniimtpe-1425Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESВлияние интенсивных физических нагрузок на биохимические показатели систем антиоксидантной защиты и оксида азота у спортсменов-пловцовThe effect of intense physical exertion on the biochemical parameters of antioxidant protection systems and nitric oxide in swimming athletesБлиноваТ. В.BlinovaT. V.

Блинова Татьяна Владимировна, вед. науч. сотр. клинического отдела

д-р мед. наук

Tatyana V. Blinova, leading researcher of clinical department

Dr. of Sci. (Med.)

btvdn@yandex.ruСтраховаЛ. А.StrakhovaL. A.КолесовС. А.KolesovS. A.ФБУН «Нижегородский научно-исследовательский институт гигиены и профпатологии» РоспотребнадзораРоссияNizhny Novgorod research institute for hygiene and occupational pathologyRussian Federation201912112019110860865Copyright © Блинова Т.В., Страхова Л.А., Колесов С.А., 20192019Блинова Т.В., Страхова Л.А., Колесов С.А.Blinova T.V., Strakhova L.A., Kolesov S.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.journal-irioh.ru/jour/article/view/1425Введение

Введение. Интенсивные физические нагрузки приводят к нарушению гомеостаза, который поддерживается сбалансированной работой систем защиты организма от оксидативного стресса, в частности, антиоксидантной, тиолдисульфидной и системой оксида азота. Исследование данных показателей позволит улучшить механизмы адаптации организма человека к физическим нагрузкам.

Цель исследования — выявить особенности изменений систем антиоксидантной защиты и оксида азота при интенсивных физических нагрузках у спортсменов-пловцов.

Материалы и методы

Материалы и методы. Исследована кровь спортсменов-пловцов (n=30, возраст 23,0±2,5 года) в условиях интенсивных физических нагрузок. Оксидативный стресс, общая антиоксидантная способность сыворотки, уровни глутатиона и метаболитов оксида азота изучались фотометрическими биохимическими методами.

Результаты

Результаты. У 73,3% спортсменов после интенсивных физических нагрузок сохранялся повышенный уровень окисленного глутатиона, у 30% наблюдался высокий уровень оксидативного стресса, у 26,7% уровень антиоксидантной способности сыворотки низкий, у 73,3% была снижена относительно начального уровня концентрация метаболитов оксида азота.

Заключение

Заключение. Интенсивная физическая активность приводит к оксидативному стрессу и нарушениям в антиоксидантной системе организма спортсмена. С увеличением нагрузки степень нарушений возрастает. Увеличивается уровень оксидативного стресса, снижается общая антиоксидантная способность сыворотки, нарушается функционирование глутатиона. Интегральные показатели оксидативного стресса, а также фракции глутатиона можно рекомендовать для мониторинга состояния оксидативного стресса.

Introduction

Introduction. Intense physical activity leads to a violation of homeostasis, which is supported by a balanced work of the body’s defense systems against oxidative stress, in particular, antioxidant, thiol disulfide and nitric oxide system. The study of these indicators will improve the mechanisms of adaptation of the human body to physical activity.

The aim of the study was to identify the features of changes in antioxidant protection systems and nitric oxide during intense physical activity in athletes-swimmers.

Materials and methods

Materials and methods. Blood of athletes-swimmers (n=30, age 23,0±2,5 years) in the conditions of intensive physical loadings is investigated. Oxidative stress, total serum antioxidant capacity, glutathione levels and nitric oxide metabolites were studied by photometric biochemical methods.

Results

Results. In 73.3% of athletes after intense physical activity remained elevated levels of oxidized glutathione, 30% had a high level of oxidative stress, 26.7% of the level of antioxidant capacity of serum is low, 73.3% was reduced relative to the initial level of the concentration of nitric oxide metabolites.

Conclusion

Conclusion. Intense physical activity leads to oxidative stress and disturbances in the antioxidant system of the athlete’s body. With increasing load, the degree of violations increases. The level of oxidative stress increases, the total antioxidant capacity of serum decreases, the functioning of glutathione is disrupted. Integral indicators of oxidative stress, as well as glutathione fractions can be recommended for monitoring the state of oxidative stress.

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The authors declare that there are no conflicts of interest present.