zurniimtpeМедицина труда и промышленная экологияRussian Journal of Occupational Health and Industrial Ecology1026-94282618-8945FSBSI “Izmerov Research Institute of Occupational Health”10.31089/1026-9428-2019-59-7-395-399zurniimtpe-1384Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESМолекулярно-генетический анализ полиморфизма генов репарации двунитевых разрывов ДНК и репарации «несоответствий» ДНК у работников вредных и опасных отраслей промышленностиMolecular genetic analysis of the polymorphism of repair genes of double-strand breaks DNA strand breaks and repair «inconsistencies» DNA in workers of hazardous industriesАндрущенкоТ. А.AndrushchenkoТatyana A.ГончаровС. В.GoncharovSergey V.ДосенкоВ. Е.DosenkoViktor Е.

Досенко Виктор Евгеньевич, проф., зав. отделом общей и молекулярной патофизиологии, д-рмед. наук. ул. Богомольца, 4, г. Киев, Украина, 01024

dosenko@biph.kiev.uaИщейкинК. Е.IscheikinKonstantin E.Государственное Учреждение «Институт медицины труда имени Ю.И. Кундиева Национальной академии медицинских наук Украины»РоссияKundiiev Institute of Occupational Health of the National Academy of Medical Sciences of UkraineRussian FederationИнститут физиологии имени А.А. Богомольца Национальной академии наук УкраиныРоссияBogomolets Institute of Physiology of the National Academy of Sciences of UkraineRussian FederationВГУЗ Украины «Украинская медицинская стоматологическая академия»Россия3Ukrainian Medical Dental AcademyRussian Federation20191608201917395399Copyright © Андрущенко Т.А., Гончаров С.В., Досенко В.Е., Ищейкин К.Е., 20192019Андрущенко Т.А., Гончаров С.В., Досенко В.Е., Ищейкин К.Е.Andrushchenko Т.A., Goncharov S.V., Dosenko V.Е., Ischeikin K.E.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.journal-irioh.ru/jour/article/view/1384

Введение. Представлены результаты исследования полиморфизмов генов репарации двунитевых разрывов ДНК:XRCC7 (rs7003908), АТМ (rs664677), репарации «несоответствий» ДНК — MLH1 (rs1799977) у шахтеров и работников асбестоцементных заводов с профессионально обусловленной бронхолегочной патологией. Цель исследования  — изучить распределение частот генотипов генов репарации ДНК: XRCC7 (rs7003908), АТМ (rs664677) и MLH1 (rs1799977) у работников вредных и опасных отраслей промышленности для выявления маркеров повышенного риска развития бронхолегочной патологии. Материалы и методы. У 90 человек с бронхолегочной патологией и 124 респондентов, которые работали в тех же условиях труда, но в анамнезе не имели заболеваний дыхательной системы, методом полимеразной цепной реакции в реальном времени изучен полиморфизм генов репарации ДНК: XRCC7 (rs7003908), АТМ (rs664677) и MLH1(rs1799977). Результаты. Установлено, что генотипы АТМ×Т/Т и MLH1×А/G ассоциированы с риском развития бронхолегочной патологии. Также установлены генотипы, которые способствуют резистентности к развитию патологии дыхательной системы: АТМ×А/А, АТМ× А/Т и MLH1×А/А. Заключение. Установлены генотипы, ассоциированные с риском развития бронхолегочной патологии: АТМ×Т/Т (р≤0,01,χ2=6,61; OR=2,48; 95%CI: 1,16–5,31) и MLH1×А/G (р≤0,002, χ2=9,00; OR=2,32; 95%CI: 1,29–4,21). Также определены генотипы, способствующие резистентности к развитию заболеваний дыхательной системы: АТМ×А/А(OR=0,83; 95%CI: 0,45–1,54), АТМ×А/Т (OR=0,67; 95%CI: 0,38–1,21) и MLH1× А/А (р≤0,003, χ2=8,73; OR=0,43; 95%CI: 0,24–0,79)

Introduction. Presents results of a study of polymorphisms of repair genes of double-strand breaks DNA breaks: XRCC7 (rs7003908), ATM (rs664677), repair «inconsistencies» DNA MLH1 (rs1799977) in miners and workers of asbestos factories professionally due to broncho-pulmonary pathology. T e aim of the study was to research the frequency distribution of genotypes of DNA repair genes: XRCC7 (rs7003908), ATM (rs664677) and MLH1 (rs1799977) in workers of harmful and dangerous industries to identify markers of increased risk of bronchopulmonary pathology. Materials and methods. In 90 people with bronchopulmonary pathology and 124 respondents who worked in the same working conditions but had no history of diseases of the respiratory system, polymerase chain reaction in real time studied the polymorphism of DNA repair genes: XRCC7 (rs7003908), ATM (rs664677) and MLH1 (rs1799977). Results. It was found that the genotypes ATM×T/T and MLH1×A/G are associated with the risk of bronchopulmonary pathology. Genotypes that contribute to resistance to the development of respiratory system pathology were also established: ATM×A/A, ATM× A/T and MLH1×A/A. Conclusion. Genotypes associated with the risk of bronchopulmonary pathology were established: ATM×T/T (р≤0.01, χ2=6.61; OR=2.48; 95%CI: 1.16–5.31) and MLH1×A/G (p≤0.002, χ2=9.00; OR=2.32; 95%CI: 1.29–4.21). Also determined the genotypes that contribute to resistance to the development of diseases of the respiratory system: ATM×a/A (OR=0,83; 95%CI: 0,45–1,54), ATM×A/T (OR=0,67; 95% CI: 0,38–1,21) and MLH1× a/A (р≤0,003, χ2=8,73; OR=0,43; 95% CI: 0,24–0,79).

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The authors declare that there are no conflicts of interest present.