zurniimtpeМедицина труда и промышленная экологияRussian Journal of Occupational Health and Industrial Ecology1026-94282618-8945FSBSI “Izmerov Research Institute of Occupational Health”10.31089/1026-9428-2019-1-9-14zurniimtpe-1303Research ArticleСтатьиОсобенности образования антител против бензо[а]пирена у работников угольных шахт и теплоэлектростанцийFeatures of antibodies against benzo[a]pyrene formation in workers of coal mines and thermal power plantsПоленокЕ. Г.PolenokYe. G.

Ленинградский пр-т, 10, Кемерово, Россия, 650065.

10, Leningradsky Ave., Kemerovo, Russia, 650065.

МунС. А.MunS. A.

Мун Стелла Андреевна, ст. науч. сотр. лаб. иммуногенетики Института экологии человека, канд. мед. наук.

Stella A. Mun, Senior Researcher of Immune Genetics Laboratory in Human Ecology Institute.

10, Leningradsky Ave., Kemerovo, Russia, 650065.

ihe@list.ruГордееваЛ. А.GordeevaL. A.

Ленинградский пр-т, 10, Кемерово, Россия, 650065.

10, Leningradsky Ave., Kemerovo, Russia, 650065.

ГлушковА. А.GlushkovA. A.

ул. Пирогова, 1, Новосибирск, Россия, 630090.

1, Pirogova str., Novosibirsk, Russia, 630090.

КостянкоМ. В.KostyankoM. V.

Красная ул., 6, Кемерово, Россия, 650000.

6, Krasnaya str.,Kemerovo, Russia, 650000.

ВафинИ. А.VafinI. A.

пр-т Октябрьский, 22, Кемерово, Россия, 650066.

22, Oktyabr’sky Ave., Kemerovo, Russia, 650066.

ФГБНУ «Федеральный исследовательский центр угля и углехимии» СО РАН.РоссияFederal Research Center of Coal and Coal Chemistry.Russian FederationФГАОУ ВО «Новосибирский национальный исследовательский государственный университет», Институт медицины и психологии.РоссияNovosibirsk State University.Russian FederationФГБОУ ВО «Кемеровский государственный университет».РоссияKemerovo State University.Russian FederationГКУЗ «Кемеровский областной центр крови».РоссияKemerovo regional blood Center.Russian Federation20191203201901914Copyright © Поленок Е.Г., Мун С.А., Гордеева Л.А., Глушков А.А., Костянко М.В., Вафин И.А., 20192019Поленок Е.Г., Мун С.А., Гордеева Л.А., Глушков А.А., Костянко М.В., Вафин И.А.Polenok Y.G., Mun S.A., Gordeeva L.A., Glushkov A.A., Kostyanko M.V., Vafin I.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.journal-irioh.ru/jour/article/view/1303

Введение. Угольная пыль и продукты сжигания угля содержат большое количество канцерогенных веществ (в частности бензо[а]пирен), которые по-разному воздействует на работников угольных шахт и угольных теплоэлектростанций. Следовательно, и специфические иммунные реакции на бензо[а]пирен у этих категорий рабочих могут иметь свои характерные особенности.Цель исследования — выявить особенности образования антител (АТ), специфичных к бензо[а]пирену, у работни-ков угольных шахт и теплоэнергетиков.Материалы и методы. Исследованы АТ классов А и G против бензо[а]пирена (IgA-Bp и IgG-Bр) в сыворотке крови у 705 мужчин: 213 доноров Кемеровского центра крови (1 группа, контроль); 293 шахтеров (2 группа) и 199 тепло-энергетиков (3 группа) с помощью иммуноферментного анализа, при использовании конъюгата Bp с бычьим сывороточным альбумином в качестве адсорбированного антигена.Результаты. Уровни IgA-Bp у шахтеров (Ме=2,7) не отличались от таковых в контрольной группе (Ме=2,9), а у тепло-энергетиков (Ме=3,7) статистически значимо превышали таковые у здоровых мужчин и у шахтеров (р<0,0001). Уровни IgG-Bр у шахтеров (Me=5,0) оказались ниже, чем в контрольной группе (Me=6,4; р=0,005). Содержание IgG-Bр у те-плоэнергетиков (Me=7,4) превышало показатели здоровых доноров и шахтеров (р<0,0001). Непроизводственные фак-торы (возраст и курение) не влияли на специфические иммунные реакции против Bp у шахтеров и теплоэнергетиков.Выводы: Специфические иммунные реакции на Bp у шахтеров и теплоэнергетиков имеют присущие им особенности: для шахтеров характерно снижение уровней сывороточных АТ класса А к Вр; для теплоэнергетиков характерно повышение уровней сывороточных АТ класса G к Вр. Эти особенности обусловлены только принадлежностью к указанным профессиям и не зависят от таких факторов, как возраст, курение и стаж работы во вредном производстве. Целесообразно исследовать особенности специфических иммунных реакций на Bp у работников угольных шахт и те плоэлектростанций при возникновении злокачественных опухолей для оценки индивидуальных онкорисков.

Introduction. Coal dust and coal fi ring products contain large amounts of carcinogenic chemicals (specifically benz[a]pyrene) that are different in influence on workers of coal mines and thermal power plants. Specific immune reactions to benz[a]pyrene therefore in these categories of workers can have specific features.Objective. To reveal features of antibodies specifi c to benz[a]pyrene formation in workers of coal mines and thermal power plants.Materials and methods. The study covered A and G class antibodies against benz[a]pyrene (IgA-Bp and IgG-Bp) in serum of 705 males: 213 donors of Kemerovo blood transfusion center (group 1, reference); 293 miners(group 2) and 199 thermal power plant workers (group 3). Benz[a]pyrene conjugate with bovine serum albumin as an adsorbed antigen was subjected to immune-enzyme assay.Results. IgA-Bp levels in the miners (Me = 2.7) did not differ from those in the reference group (Me = 2.9), but in the thermal power plant workers (Me = 3.7) were reliably higher than those in healthy men and in the miners (p<0.0001). Levels of IgG-Bp in the miners (Me = 5.0) appeared to be lower than those in the reference group (Me = 6.4; (p = 0.05). IgG-Bb level in the thermal power plantworkers (Me = 7.4) exceeded the parameters in the healthy donors and the miners (p<0.0001). Non-industrial factors (age and smoking) appeared tohave no influence on specific immune reactions against benz[a]pyrene in the miners and the thermal power plant workers.Conclusions. Specific immune reactions against benz[a]pyrene in the miners and the thermal power plant workers are characterized by peculiarities: the miners demonstrate lower levels of class A serum antibodies to benz[a]pyrene; the thermal power plant workers present increased serum levels of class G antibodies to benz[a]pyrene. These peculiarities result from only the occupational features, but do not depend on such factors as age, smoking and length of service at hazardous production. It is expedient to study specific immune reactions to benz[a]pyrene in workers of coal mines and thermal power plants, to evaluate individual oncologic risk and if malignancies occur.

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The authors declare that there are no conflicts of interest present.